Development and Quantitative Determination of Barakol in Senna Siamea Leaf Extract by Tlc-image Analysis Method Apirak Sakunpak*, Jirapornchai Suksaeree, Chaowalit Monton and Pathamaporn Pathompak

Objective: The aim of this study was to develop and validate an image analysis method for quantitative analysis of barakol content in Senna siamea leaf extract. Methods: TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of Senna siamea leaf extract. The results obtained by these two different quantification methods were compared by paired t-test. Results: Both assays provided good linearity, accuracy, reproducibility, and selectivity for determination of barakol in Senna siamea leaf extract. Conclusions: The TLC-densitometric and TLC-image analysis methods provided a good reproducibility, accuracy and selectivity for the quantitative determination of barakol in Senna siamea leaf extract. A statistical comparison of the quantitative analysis of barakol in samples did not show any statistically significant difference between two analysis methods. As both methods were found to be equal, they therefore can be used for the analysis of barakol content in Senna siamea leaf extract. Keyword: Quantitative, Barakol, Senna siamea, Image-analysis, TLC-densitrometric